Transcripts
DR. LINGWOOD:
The Gb3 cell surface can be
organized in microdomains or lipid rafts. A
lipid raft is shown here. Now these have been
described for some time. And it's a sort of a
blob on the cell surface. It's a domain, an
enriched domain of different lipids, lipids that
are different from the bulk lipid structure here
shown in yellow. So this pinky yellowy area is
the lipid raft, which is a series of lipids
which tend to float around on the cell surface.
In fact, it doesn't diffuse as these do across
the cell surface. And so what I'm showing here
or what has been shown are these lipid rafts are
enriched in what are called GPI anchored proteins
oftentimes signaling -- I won't go into that --
what those are.
But what they're really enriched
in is cholesterol here in green and glycolipids
show here as these red balls. And what I've
put here is Gb3 question mark. And Gb3
definitely is in these rafts in some cells, in
the cells that target VT to the retrograde
transit pathway.
The Gb3 is not in this domain
in the cells which target the endosomal pathway
to stimulate cell synthesis. The Gb3 is not in
these domains, it's just diffusely spread in the plasma lipid.
Characterization in terms of lipid
structure is different, can be also different.
So we have another degree of
complexity in terms of verotoxin induced
pathology and that is the receptor organization
on the cell surface.
